PacBio sequencing submission form

Please complete the form below and click the "Submit" button to make your submission. Required fields are marked with an asterisk (*). If you have any questions related to the submission form, please send an email to seq-team@sf.czbiohub.org.

Before filling out this form, please be sure you have read the instructions on the previous page.

Submission information

Principal Investigator first name **

Principal Investigator last name **

Submitter first name **

Submitter last name **

Enter email addresses to receive results (one email per line) **

Please enter one email address per line

Institute **

PI project task code **

This can be acquired from the affiliated PI

Study description (12 words minimum) **

CZB Metadata Project ID (optional; CZB internal use only)

CZB Metadata Experiment ID (optional; CZB internal use only)

Library information

Library type **

Nucleic acid extraction method **

Library pool tube label **

Please label the tube with the same library pool tube name provided here

Library pool concentration (ng/ul or nM) **

Library insert size (bp) - 100 to 50,000 **

Library volume (μL) - ≥ 14 **

Number of libraries in pool - 1 to 1000 **

Sequencing run information

Sequencing platform (run costs refer here) **

Number of SMRT Cells to load **

Select the number of SMRT cells needed (one SMRT Cell 8M will yield 20-30 Gb)

Sequencing application*

Sequencing application details

If the sequencing application is different from the recommendation, please complete the optional fields below.

Movie time (in hours)

Please enter the movie time if different from the application recommendation

Additional information

Select analysis applications **

CCS

Demultiplexed barcodes

Additional comments (optional)

Library trace and barcode sample sheet uploads

Library trace and barcode metadata

Library trace method **

Library trace notes

Barcode set

Library trace upload

Upload library trace file (only PDF files are supported) **

Note: due to security concerns, you might need to re-upload the file in case of an error in the form.

Barcode sample sheet upload

The template for the sample sheet can be found here.
When submitting your sample sheet, please do not include any personally identifiable data or personal health information.

Upload barcode sample sheet (only CSV files are supported) *.*

Note: due to security concerns, you might need to re-upload the file in case of an error in the form.

Submit

When you are ready to make your submission, click the 'submit' button below. Note that once you submit this form, you will not be able to edit your submission. Please double-check that all of the information entered above is correct before clicking 'submit'.

If your submission is successful, you should see a new page that says "Submission successful." If you do not see this page, please review this form for any error messages, correct them, and then try to submit the form again.